NUMA1 Antibody | CSB-PA016185YA01HU

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CSB-PA016185YA01HU
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  • NUMA1 Antibody
  • Western Blot<br />
 Positive WB detected in: Hela whole cell lysate, K562 whole cell lysate<br />
 All lanes: NUMA1 antibody at 3.2µg/ml<br />
 Secondary<br />
 Goat polyclonal to rabbit IgG at 1/50000 dilution<br />
 Predicted band size: 239, 237, 202, 201, 110 kDa<br />
 Observed band size: 239 kDa<br />
  • Immunohistochemistry of paraffin-embedded human breast cancer using CSB-PA016185YA01HU at dilution of 1:100
  • Immunohistochemistry of paraffin-embedded human thyroid tissue using CSB-PA016185YA01HU at dilution of 1:100
  • Immunofluorescence staining of MCF-7 cells with CSB-PA016185YA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L) .
€315.80 - €488.70

Description

NUMA1 Antibody | CSB-PA016185YA01HU | Cusabio

NUMA1 Antibody is Available at Gentaur Genprice with the fastest delivery.

Online Order Payment is possible or send quotation to info@gentaur.com.

Product Type: Polyclonal Antibody

Target Names: NUMA1

Aliases: Nuclear mitotic apparatus protein 1 (Nuclear matrix protein-22) (NMP-22) (Nuclear mitotic apparatus protein) (NuMA protein) (SP-H antigen), NUMA1, NMP22 NUMA

Background: Highly abundant component of the nuclear matrix where it may serve a non-mitotic structural role, occupies the majority if the nuclear volume (PubMed:10075938) . Required for maintenance and establishment of the mitotic spindle poles during symmetric cell divisions, functioning as a tether linking bulk microtubules of the spindle to centrosomes (PubMed:7769006, PubMed:11956313, PubMed:26195665) . Also required for proper alignment of the mitotic spindle during asymmetric cell divisions (PubMed:21816348) .

Isotype: IgG

Conjugate: Non-conjugated

Clonality: Polyclonal

Uniport ID: Q14980

Host Species: Rabbit

Species Reactivity: Human

Immunogen: Recombinant Human Nuclear mitotic apparatus protein 1 protein (627-870AA)

Immunogen Species: Human

Applications: ELISA, WB, IHC, IF

Tested Applications: ELISA, WB, IHC, IF; Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200

Purification Method: >95%, Protein G purified

Dilution Ratio1: ELISA:1:2000-1:10000

Dilution Ratio2: WB:1:500-1:5000

Dilution Ratio3: IHC:1:20-1:200

Dilution Ratio4: IF:1:50-1:200

Dilution Ratio5:

Dilution Ratio6:

Buffer: Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form: Liquid

Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Initial Research Areas: Cell Biology

Research Areas: Cell biology

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