Mono-methyl-HIST1H1C (K45) Antibody | CSB-PA010378OA45me1HU

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CSB-PA010378OA45me1HU
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  • Mono-methyl-HIST1H1C (K45) Antibody
  • Western Blot<br />
 Positive WB detected in: 293 whole cell lysate, A549 whole cell lysate<br />
 All lanes: HIST1H1C antibody at 1:100<br />
 Secondary<br />
 Goat polyclonal to rabbit IgG at 1/50000 dilution<br />
 Predicted band size: 22 kDa<br />
 Observed band size: 22 kDa<br />
  • Immunocytochemistry analysis of CSB-PA010378OA45me1HU diluted at 1:25 and staining in Hela cells performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
  • Immunofluorescence staining of Hela cells with CSB-PA010378OA45me1HU at 1:12.5, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L) .
€327.50 - €514.70

Description

Mono-methyl-HIST1H1C (K45) Antibody | CSB-PA010378OA45me1HU | Cusabio

Mono-methyl-HIST1H1C (K45) Antibody is Available at Gentaur Genprice with the fastest delivery.

Online Order Payment is possible or send quotation to info@gentaur.com.

Product Type: Polyclonal Antibody

Target Names: HIST1H1C

Aliases: Histone H1.2 (Histone H1c) (Histone H1d) (Histone H1s-1), HIST1H1C, H1F2

Background: Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation (By similarity) .

Isotype: IgG

Conjugate: Non-conjugated

Clonality: Polyclonal

Uniport ID: P16403

Host Species: Rabbit

Species Reactivity: Human

Immunogen: Peptide sequence around site of Mono-methyl-Lys (45) derived from Human Histone H1.2

Immunogen Species: Human

Applications: ELISA, WB, ICC, IF

Tested Applications: ELISA, WB, ICC, IF; Recommended dilution: WB:1:100-1:1000, ICC:1:20-1:200, IF:1:10-1:100

Purification Method: Antigen Affinity Purified

Dilution Ratio1: ELISA:1:2000-1:10000

Dilution Ratio2: WB:1:100-1:1000

Dilution Ratio3: ICC:1:20-1:200

Dilution Ratio4: IF:1:10-1:100

Dilution Ratio5:

Dilution Ratio6:

Buffer: Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form: Liquid

Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Initial Research Areas: Epigenetics and Nuclear Signaling

Research Areas: Epigenetics & Nuclear Signaling

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