Histone H3K4me2 Antibody | CSB-PA087078

Histone H3K4me2 Antibody | CSB-PA087078 | Cusabio

Histone H3K4me2 Antibody is Available at Gentaur Genprice with the fastest delivery.

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Product Type: Polyclonal Antibody

Target Names: HIST3H3

Aliases: HIST1H3J; H3/j;H3FJ; Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/I; HistoneH3/j; Histone H3/k; Histone H3/l; HIST3H3;

Background: Modulation of chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin (1) . The amino-terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination (2-5) . These modifications occur in response to various stimuli and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, gene expression (6) . In most species, histone H2B is primarily acetylated at Lys5, 12, 15, and 20 (4, 7) . Histone H3 is primarily acetylated at Lys9, 14, 18, 23, 27, and 56. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (2, 3) . Phosphorylation at Ser10, Ser28, and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis (8-10) . Phosphorylation at Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kinase haspin. Immunostaining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation at Thr3 of H3 in prophase and its dephosphorylation during anaphase (11) .

Isotype: IgG

Conjugate: Non-conjugated

Clonality: Polyclonal

Uniport ID: Q16695

Host Species: Rabbit

Species Reactivity: Human, Mouse, Rat

Immunogen: A synthetic methylated peptide corresponding to residues surrounding K4 of human histone H3

Immunogen Species: Human

Applications: ELISA, WB, IHC, IF, IP, ChIP

Tested Applications: ELISA, WB, IHC, IF, IP, ChIP;WB:1:500-1:2000, IHC:1:50-1:200, IF:1:50-1:200, IP:1:50-1:200, ChIP:1:50-1:200

Purification Method: Antigen Affinity Purified

Dilution Ratio1: ELISA:1:2000-1:10000

Dilution Ratio2: WB:1:500-1:2000

Dilution Ratio3: IHC:1:50-1:200

Dilution Ratio4: IF:1:50-1:200

Dilution Ratio5: IP:1:50-1:200

Dilution Ratio6: ChIP:1:50-1:200

Buffer: Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Form: liquid

Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Initial Research Areas: Epigenetics and Nuclear Signaling

Research Areas: Epigenetics & Nuclear Signaling