HIST1H3A (Ab-128) Antibody | CSB-PA010418OA128nme1HU

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CSB-PA010418OA128nme1HU
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  • HIST1H3A (Ab-128) Antibody
  • IHC image of CSB-PA010418OA128nme1HU diluted at 1:20 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
  • IHC image of CSB-PA010418OA128nme1HU diluted at 1:20 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
  • Western Blot<br />
 Positive WB detected in: Hela whole cell lysate, Jurkat whole cell lysate, 293 whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysate, Rat liver tissue, Rat kidney tissue, Mouse brain tissue, Mouse liver tissue<br />
 All lanes: HIST1H3A antibody at 0.53µg/ml<br />
 Secondary<br />
 Goat polyclonal to rabbit IgG at 1/50000 dilution<br />
 Predicted band size: 16 kDa<br />
 Observed band size: 16 kDa<br />
  • Immunoprecipitating HIST1H3A in Hela whole cell lysate<br />
 Lane 1: Rabbit control IgG instead of CSB-PA010418OA128nme1HU in Hela whole cell lysate.
 For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) <br />
 Lane 2: CSB-PA010418OA128nme1HU (5µg) + Hela whole cell lysate (500µg) <br />
 Lane 3: Hela whole cell lysate (20µg) <br />
€327.50 - €514.70

Description

HIST1H3A (Ab-128) Antibody | CSB-PA010418OA128nme1HU | Cusabio

HIST1H3A (Ab-128) Antibody is Available at Gentaur Genprice with the fastest delivery.

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Product Type: Polyclonal Antibody

Target Names: HIST1H3A

Aliases: Histone H3.1 (Histone H3/a) (Histone H3/b) (Histone H3/c) (Histone H3/d) (Histone H3/f) (Histone H3/h) (Histone H3/i) (Histone H3/j) (Histone H3/k) (Histone H3/l), HIST1H3A; HIST1H3B; HIST1H3C; HIST1H3D; HIST1H3E; HIST1H3F; HIST1H3G; HIST1H3H; HIST1H3I; HIST1H3J, H3FA; H3FL; H3FC; H3FB; H3FD; H3FI; H3FH; H3FK; H3FF; H3FJ

Background: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Isotype: IgG

Conjugate: Non-conjugated

Clonality: Polyclonal

Uniport ID: P68431

Host Species: Rabbit

Species Reactivity: Human, Mouse, Rat

Immunogen: Peptide sequence around site of Arg (128) derived from Human Histone H3.1

Immunogen Species: Human

Applications: ELISA, WB, IHC, IP

Tested Applications: ELISA, WB, IHC, IP; Recommended dilution: WB:1:200-1:2000, IHC:1:10-1:100, IP:1:200-1:2000

Purification Method: Antigen Affinity Purified

Dilution Ratio1: ELISA:1:2000-1:10000

Dilution Ratio2: WB:1:200-1:2000

Dilution Ratio3: IHC:1:10-1:100

Dilution Ratio4: IP:1:200-1:2000

Dilution Ratio5:

Dilution Ratio6:

Buffer: Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form: Liquid

Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Initial Research Areas: Epigenetics and Nuclear Signaling

Research Areas: Epigenetics & Nuclear Signaling

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