HIST1H1E (Ab-45) Antibody | CSB-PA010380OA45nacHU

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SKU:
CSB-PA010380OA45nacHU
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  • HIST1H1E (Ab-45) Antibody
  • Western Blot<br />
 Positive WB detected in: 293 whole cell lysate<br />
 All lanes: HIST1H1E antibody at 0.94µg/ml<br />
 Secondary<br />
 Goat polyclonal to rabbit IgG at 1/50000 dilution<br />
 Predicted band size: 22 kDa<br />
 Observed band size: 22 kDa<br />
  • IHC image of CSB-PA010380OA45nacHU diluted at 1:5 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
  • IHC image of CSB-PA010380OA45nacHU diluted at 1:5 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
  • Immunofluorescence staining of Hela cells with CSB-PA010380OA45nacHU at 1:2.5, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L) .
€327.50 - €514.70

Description

HIST1H1E (Ab-45) Antibody | CSB-PA010380OA45nacHU | Cusabio

HIST1H1E (Ab-45) Antibody is Available at Gentaur Genprice with the fastest delivery.

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Product Type: Polyclonal Antibody

Target Names: HIST1H1E

Aliases: Histone H1.4 (Histone H1b) (Histone H1s-4), HIST1H1E, H1F4

Background: Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation (By similarity) .

Isotype: IgG

Conjugate: Non-conjugated

Clonality: Polyclonal

Uniport ID: P10412

Host Species: Rabbit

Species Reactivity: Human

Immunogen: Peptide sequence around site of Lys (45) derived from Human Histone H1.4

Immunogen Species: Human

Applications: ELISA, WB, IHC, IF

Tested Applications: ELISA, WB, IHC, IF; Recommended dilution: WB:1:100-1:1000, IHC:1:1-1:10, IF:1:1-1:10

Purification Method: Antigen Affinity Purified

Dilution Ratio1: ELISA:1:2000-1:10000

Dilution Ratio2: WB:1:100-1:1000

Dilution Ratio3: IHC:1:1-1:10

Dilution Ratio4: IF:1:1-1:10

Dilution Ratio5:

Dilution Ratio6:

Buffer: Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form: Liquid

Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Initial Research Areas: Epigenetics and Nuclear Signaling

Research Areas: Epigenetics & Nuclear Signaling

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