H1F0 (Ab-101) Antibody | CSB-PA010087OA101nme1HU

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CSB-PA010087OA101nme1HU
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3 to 7 Working Days
  • H1F0 (Ab-101) Antibody
  • Western Blot<br />
 Positive WB detected in: Rat spleen tissue<br />
 All lanes: H1F0 antibody at 0.85µg/ml<br />
 Secondary<br />
 Goat polyclonal to rabbit IgG at 1/50000 dilution<br />
 Predicted band size: 21, 20 kDa<br />
 Observed band size: 21 kDa<br />
  • IHC image of CSB-PA010087OA101nme1HU diluted at 1:50 and staining in paraffin-embedded human melanoma performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
  • Immunofluorescence staining of HepG2 cells with CSB-PA010087OA101nme1HU at 1:1, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L) .
  • Chromatin Immunoprecipitation Hela (4*10<sup>6</sup>) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5µg anti-H1F0 (CSB-PA010087OA101nme1HU) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter.
€327.50 - €514.70

Description

H1F0 (Ab-101) Antibody | CSB-PA010087OA101nme1HU | Cusabio

H1F0 (Ab-101) Antibody is Available at Gentaur Genprice with the fastest delivery.

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Product Type: Polyclonal Antibody

Target Names: H1F0

Aliases: Histone H1.0 (Histone H1') (Histone H1 (0) ) [Cleaved into: Histone H1.0, N-terminally processed], H1F0, H1FV

Background: Histones H1 are necessary for the condensation of nucleosome chains into higher-order structures. The H1F0 histones are found in cells that are in terminal stages of differentiation or that have low rates of cell division.

Isotype: IgG

Conjugate: Non-conjugated

Clonality: Polyclonal

Uniport ID: P07305

Host Species: Rabbit

Species Reactivity: Human, Rat

Immunogen: Peptide sequence around site of Lys (101) derived from Human Histone H1.0

Immunogen Species: Human

Applications: ELISA, WB, IHC, IF, ChIP

Tested Applications: ELISA, WB, IHC, IF, ChIP; Recommended dilution: WB:1:50-1:500, IHC:1:20-1:200, IF:1:1-1:10

Purification Method: Antigen Affinity Purified

Dilution Ratio1: ELISA:1:2000-1:10000

Dilution Ratio2: WB:1:50-1:500

Dilution Ratio3: IHC:1:20-1:200

Dilution Ratio4: IF:1:1-1:10

Dilution Ratio5:

Dilution Ratio6:

Buffer: Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form: Liquid

Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Initial Research Areas: Epigenetics and Nuclear Signaling

Research Areas: Epigenetics & Nuclear Signaling

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