GOLPH3 Antibody | CSB-PA872494LA01HU

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SKU:
CSB-PA872494LA01HU
Availability:
3 to 7 Working Days
  • GOLPH3 Antibody
  • Western Blot<br />
 Positive WB detected in: Hela whole cell lysate, 293 whole cell lysate, A549 whole cell lysate<br />
 All lanes: GOLPH3 antibody at 3.2µg/ml<br />
 Secondary<br />
 Goat polyclonal to rabbit IgG at 1/50000 dilution<br />
 Predicted band size: 34 kDa<br />
 Observed band size: 34 kDa<br />
  • Immunohistochemistry of paraffin-embedded human prostate cancer using CSB-PA872494LA01HU at dilution of 1:100
  • Immunohistochemistry of paraffin-embedded human prostate tissue using CSB-PA872494LA01HU at dilution of 1:100
  • Immunofluorescence staining of HepG2 cells with CSB-PA872494LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L) .
  • Immunoprecipitating GOLPH3 in Hela whole cell lysate<br />
 Lane 1: Rabbit control IgG instead of CSB-PA872494LA01HU in Hela whole cell lysate.
 For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) <br />
 Lane 2: CSB-PA872494LA01HU (8µg) + Hela whole cell lysate (500µg) <br />
 Lane 3: Hela whole cell lysate (10µg) <br />
€315.80 - €488.70

Description

GOLPH3 Antibody | CSB-PA872494LA01HU | Cusabio

GOLPH3 Antibody is Available at Gentaur Genprice with the fastest delivery.

Online Order Payment is possible or send quotation to info@gentaur.com.

Product Type: Polyclonal Antibody

Target Names: GOLPH3

Aliases: Golgi phosphoprotein 3 (Coat protein GPP34) (Mitochondrial DNA absence factor) (MIDAS), GOLPH3, GPP34

Background: Phosphatidylinositol-4-phosphate-binding protein that links Golgi membranes to the cytoskeleton and may participate in the tensile force required for vesicle budding from the Golgi. Thereby, may play a role in Golgi membrane trafficking and could indirectly give its flattened shape to the Golgi apparatus. May also bind to the coatomer to regulate Golgi membrane trafficking. May play a role in anterograde transport from the Golgi to the plasma membrane and regulate secretion. Has also been involved in the control of the localization of Golgi enzymes through interaction with their cytoplasmic part. May play an indirect role in cell migration. Has also been involved in the modulation of mTOR signaling. May also be involved in the regulation of mitochondrial lipids biosynthesis.

Isotype: IgG

Conjugate: Non-conjugated

Clonality: Polyclonal

Uniport ID: Q9H4A6

Host Species: Rabbit

Species Reactivity: Human

Immunogen: Recombinant Human Golgi phosphoprotein 3 protein (16-150AA)

Immunogen Species: Human

Applications: ELISA, WB, IHC, IF, IP

Tested Applications: ELISA, WB, IHC, IF, IP; Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IF:1:20-1:200, IP:1:200-1:2000

Purification Method: >95%, Protein G purified

Dilution Ratio1: ELISA:1:2000-1:10000

Dilution Ratio2: WB:1:500-1:5000

Dilution Ratio3: IHC:1:20-1:200

Dilution Ratio4: IF:1:20-1:200

Dilution Ratio5: IP:1:200-1:2000

Dilution Ratio6:

Buffer: Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form: Liquid

Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Initial Research Areas: Tags & Cell Markers

Research Areas: Tags & Cell Markers;Signal transduction

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