GAPDH Antibody | CSB-PA779821

GAPDH Antibody | CSB-PA779821 | Cusabio

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Product Type: Polyclonal Antibody

Target Names: GAPDH

Aliases: Glyceraldehyde-3-phosphate dehydrogenase

Background: The abundance of glyceraldehyde-3-phosphate dehydrogenase in eukaryotic cells is relatively unaffected by eternal factors. In western blot analysis the level of GAPDH, a 38kDa protein, can be used as a reference value for loading control. Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC By similarity. Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate.

Isotype: IgG

Conjugate: Non-conjugated

Clonality: Polyclonal

Uniport ID: P04406

Host Species: Rabbit

Species Reactivity: Human, Mouse, Rat

Immunogen: Fusion protein of human GAPDH

Immunogen Species: Human

Applications: ELISA, WB, IHC

Tested Applications: ELISA, WB, IHC;ELISA:1:1000-1:10000, WB:1:1000-1:10000, IHC:1:50-1:200

Purification Method: Antigen affinity purification

Dilution Ratio1: ELISA:1:1000-1:10000

Dilution Ratio2: WB:1:1000-1:10000

Dilution Ratio3: IHC:1:50-1:200

Dilution Ratio4:

Dilution Ratio5:

Dilution Ratio6:

Buffer: -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol

Form: Liquid

Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Initial Research Areas: Neuroscience

Research Areas: Isotype Loading Controls;Neuroscience;Cancer;Metabolism;Signal transduction