DVL2 Antibody | CSB-PA007286ESR1HU

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CSB-PA007286ESR1HU
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  • DVL2 Antibody
  • Immunohistochemistry of paraffin-embedded human lung tissue using CSB-PA007286ESR1HU at dilution of 1:100
  • Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA007286ESR1HU at dilution of 1:100
  • Western Blot<br />
 Positive WB detected in: HepG2 whole cell lysate<br />
 All lanes: DVL2 antibody at 2.3µg/ml<br />
 Secondary<br />
 Goat polyclonal to rabbit IgG at 1/50000 dilution<br />
 Predicted band size: 79 kDa<br />
 Observed band size: 79 kDa<br />
  • IHC image of CSB-PA007286ESR1HU diluted at 1:227 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
  • IHC image of CSB-PA007286ESR1HU diluted at 1:227 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
  • Immunofluorescence staining of A549 cells with CSB-PA007286ESR1HU at 1:75, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L) .
€230.00 - €317.10

Description

DVL2 Antibody | CSB-PA007286ESR1HU | Cusabio

DVL2 Antibody is Available at Gentaur Genprice with the fastest delivery.

Online Order Payment is possible or send quotation to info@gentaur.com.

Product Type: Polyclonal Antibody

Target Names: DVL2

Aliases: Segment polarity protein dishevelled homolog DVL-2 (Dishevelled-2) (DSH homolog 2), DVL2

Background: Participates in Wnt signaling by binding to the cytoplasmic C-terminus of frizzled family members and transducing the Wnt signal to down-stream effectors. Promotes internalization and degradation of frizzled proteins upon Wnt signaling. Plays a role both in canonical and non-canonical Wnt signaling. Plays a role in the signal transduction pathways mediated by multiple Wnt genes .

Isotype: IgG

Conjugate: Non-conjugated

Clonality: Polyclonal

Uniport ID: O14641

Host Species: Rabbit

Species Reactivity: Human

Immunogen: Recombinant Human Segment polarity protein dishevelled homolog DVL-2 protein (1-240AA)

Immunogen Species: Human

Applications: ELISA, WB, IHC, IF

Tested Applications: ELISA, WB, IHC, IF; Recommended dilution: WB:1:500-1:2000, IHC:1:20-1:500, IF:1:50-1:200

Purification Method: Antigen Affinity Purified

Dilution Ratio1: ELISA:1:2000-1:10000

Dilution Ratio2: WB:1:500-1:2000

Dilution Ratio3: IHC:1:20-1:500

Dilution Ratio4: IF:1:50-1:200

Dilution Ratio5:

Dilution Ratio6:

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Form: Liquid

Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Initial Research Areas: Stem Cells

Research Areas: Stem cells

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