CYP21A2 Antibody | CSB-PA006400LA01HU

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CSB-PA006400LA01HU
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  • CYP21A2 Antibody
  • Western Blot<br />
 Positive WB detected in: Jurkat whole cell lysate, HL-60 whole cell lysate, 293 whole cell lysate, SH-SY5Y whole cell lysate, Hela whole cell lysate, PC3 whole cell lysate<br />
 All lanes: CYP21A2 antibody at 1:500<br />
 Secondary<br />
 Goat polyclonal to rabbit IgG at 1/50000 dilution<br />
 Predicted band size: 56, 53 kDa<br />
 Observed band size: 56 kDa<br />
  • IHC image of CSB-PA006400LA01HU diluted at 1:100 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
  • Immunofluorescence staining of HepG2 with CSB-PA006400LA01HU at 1:33, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L) .
€315.80 - €488.70

Description

CYP21A2 Antibody | CSB-PA006400LA01HU | Cusabio

CYP21A2 Antibody is Available at Gentaur Genprice with the fastest delivery.

Online Order Payment is possible or send quotation to info@gentaur.com.

Product Type: Polyclonal Antibody

Target Names: CYP21A2

Aliases: Steroid 21-hydroxylase (EC 1.14.14.16) (21-OHase) (Cytochrome P-450c21) (Cytochrome P450 21) (Cytochrome P450 XXI) (Cytochrome P450-C21) (Cytochrome P450-C21B), CYP21A2, CYP21 CYP21B

Background: Specifically catalyzes the 21-hydroxylation of steroids. Required for the adrenal synthesis of mineralocorticoids and glucocorticoids (PubMed:22014889) .

Isotype: IgG

Conjugate: Non-conjugated

Clonality: Polyclonal

Uniport ID: P08686

Host Species: Rabbit

Species Reactivity: Human

Immunogen: Recombinant Human Steroid 21-hydroxylase protein (312-418AA)

Immunogen Species: Human

Applications: ELISA, WB, IHC, IF

Tested Applications: ELISA, WB, IHC, IF; Recommended dilution: WB:1:100-1:1000, IHC:1:20-1:200, IF:1:20-1:200

Purification Method: >95%, Protein G purified

Dilution Ratio1: ELISA:1:2000-1:10000

Dilution Ratio2: WB:1:100-1:1000

Dilution Ratio3: IHC:1:20-1:200

Dilution Ratio4: IF:1:20-1:200

Dilution Ratio5:

Dilution Ratio6:

Buffer: Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form: Liquid

Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Initial Research Areas: Cardiovascular

Research Areas: Cancer;Cardiovascular;Metabolism;Signal transduction

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