CDH1 Antibody | CSB-PA005034MA01HU

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CSB-PA005034MA01HU
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3 to 7 Working Days
  • CDH1 Antibody
  • Western Blot<br />
 Positive WB detected in: 293 whole cell lysate<br />
 All lanes: CDH1 antibody at 2.9µg/ml<br />
 Secondary<br />
 Goat polyclonal to rabbit IgG at 1/50000 dilution<br />
 Predicted band size: 98, 91 kDa<br />
 Observed band size: 125 kDa<br />
  • IHC image of CSB-PA005034MA01HU diluted at 1:300 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
  • Immunofluorescence staining of HepG2 cells with CSB-PA005034MA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L) .
  • Immunoprecipitating CDH1 in HEK293 whole cell lysate<br />
 Lane 1: Rabbit control IgG instead of CSB-PA005034MA01HU in HEK293 whole cell lysate.
 For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) <br />
 Lane 2: CSB-PA005034MA01HU (6µg) + HEK293 whole cell lysate (500µg) <br />
 Lane 3: HEK293 whole cell lysate (20µg) <br />
€315.80 - €488.70

Description

CDH1 Antibody | CSB-PA005034MA01HU | Cusabio

CDH1 Antibody is Available at Gentaur Genprice with the fastest delivery.

Online Order Payment is possible or send quotation to info@gentaur.com.

Product Type: Polyclonal Antibody

Target Names: CDH1

Aliases: Cadherin-1 (CAM 120/80) (Epithelial cadherin) (E-cadherin) (Uvomorulin) (CD antigen CD324) [Cleaved into: E-Cad/CTF1; E-Cad/CTF2; E-Cad/CTF3], CDH1, CDHE UVO

Background: Cadherins are calcium-dependent cell adhesion proteins (PubMed:11976333) . They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells (PubMed:11976333) . Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7.

Isotype: IgG

Conjugate: Non-conjugated

Clonality: Polyclonal

Uniport ID: P12830

Host Species: Rabbit

Species Reactivity: Human

Immunogen: Recombinant Human Cadherin-1 protein (23-621AA)

Immunogen Species: Human

Applications: ELISA, WB, IHC, IF, IP

Tested Applications: ELISA, WB, IHC, IF, IP; Recommended dilution: WB:1:500-1:5000, IHC:1:200-1:500, IF:1:50-1:200, IP:1:200-1:2000

Purification Method: >95%, Protein G purified

Dilution Ratio1: ELISA:1:2000-1:10000

Dilution Ratio2: WB:1:500-1:5000

Dilution Ratio3: IHC:1:200-1:500

Dilution Ratio4: IF:1:50-1:200

Dilution Ratio5: IP:1:200-1:2000

Dilution Ratio6:

Buffer: Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form: Liquid

Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Initial Research Areas: Signal Transduction

Research Areas: Cancer;Developmental biology;Signal transduction

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