Butyrly-HIST1H4A (K5) Antibody | CSB-PA010429OA05butHU

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CSB-PA010429OA05butHU
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  • Butyrly-HIST1H4A (K5) Antibody
  • Western Blot<br />
 Detected samples: Hela whole cell lysate, Jurkat whole cell lysate, 293 whole cell lysate, HepG2 whole cell lysate; Untreated (-) or treated (+) with 30mM sodium butyrate for 4h<br />
 All lanes: HIST1H4A antibody at 1:1000<br />
 Secondary<br />
 Goat polyclonal to rabbit IgG at 1/40000 dilution<br />
 Predicted band size: 12 kDa<br />
 Observed band size: 12 kDa<br />
  • Immunofluorescence staining of HepG2 cells (treated with 30mM sodium butyrate for 4h) with CSB-PA010429OA05butHU at 1:5, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L) .
  • Immunoprecipitating HIST1H4A in HepG2 whole cell lysate (treated with 30mM sodium butyrate for 4h) <br />
 Lane 1: Rabbit control IgG instead of CSB-PA010429OA05butHU in HepG2 whole cell lysate (treated with 30mM sodium butyrate for 4h) .
 For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) <br />
 Lane 2: CSB-PA010429OA05butHU (5µg) + HepG2 whole cell lysate (treated with 30mM sodium butyrate for 4h) (500µg) <br />
 Lane 3: HepG2 whole cell lysate (treated with 30mM sodium butyrate for 4h) (20µg) <br />
  • Chromatin Immunoprecipitation Hela (4*10<sup>6</sup>, treated with 30mM sodium butyrate for 4h) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5µg anti-HIST1H4A (CSB-PA010429OA05butHU) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter.
€327.50 - €514.70

Description

Butyrly-HIST1H4A (K5) Antibody | CSB-PA010429OA05butHU | Cusabio

Butyrly-HIST1H4A (K5) Antibody is Available at Gentaur Genprice with the fastest delivery.

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Product Type: Polyclonal Antibody

Target Names: HIST1H4A

Aliases: Histone H4, HIST1H4A; HIST1H4B; HIST1H4C; HIST1H4D; HIST1H4E; HIST1H4F; HIST1H4H; HIST1H4I; HIST1H4J; HIST1H4K; HIST1H4L; HIST2H4A; HIST2H4B; HIST4H4, H4/A H4FA; H4/I H4FI; H4/G H4FG; H4/B H4FB; H4/J H4FJ; H4/C H4FC; H4/H H4FH; H4/M H4FM; H4/E H4FE; H4/D H4FD; H4/K H4FK; H4/N H4F2 H4FN HIST2H4; H4/O H4FO;

Background: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Isotype: IgG

Conjugate: Non-conjugated

Clonality: Polyclonal

Uniport ID: P62805

Host Species: Rabbit

Species Reactivity: Human

Immunogen: Peptide sequence around site of Butyrly-Lys (5) derived from Human Histone H4

Immunogen Species: Human

Applications: ELISA, WB, IF, IP, ChIP

Tested Applications: ELISA, WB, IF, IP, ChIP; Recommended dilution: WB:1:500-1:2000, IF:1:1-1:10, IP:1:200-1:2000

Purification Method: Antigen Affinity Purified

Dilution Ratio1: ELISA:1:2000-1:10000

Dilution Ratio2: WB:1:500-1:2000

Dilution Ratio3: IF:1:1-1:10

Dilution Ratio4: IP:1:200-1:2000

Dilution Ratio5:

Dilution Ratio6:

Buffer: Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form: Liquid

Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Initial Research Areas: Epigenetics and Nuclear Signaling

Research Areas: Epigenetics & Nuclear Signaling

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