BUB1B Antibody | CSB-PA002883LA01HU

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SKU:
CSB-PA002883LA01HU
Availability:
3 to 7 Working Days
  • BUB1B Antibody
  • Immunohistochemistry of paraffin-embedded human endometrial cancer using CSB-PA002883LA01HU at dilution of 1:100
  • Immunofluorescent analysis of HepG2 cells using CSB-PA002883LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L)
  • Immunoprecipitating BUB1B in JK whole cell lysate <br />
 Lane 1: Rabbit control IgG (1µg) instead of CSB-PA002883LA01HU in Jurkat whole cell lysate.
 For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) <br />
 Lane 2: CSB-PA002883LA01HU (6µg) + JK whole cell lysate (500µg) <br />
 Lane 3: JK whole cell lysate (10µg) <br />
€315.80 - €488.70

Description

BUB1B Antibody | CSB-PA002883LA01HU | Cusabio

BUB1B Antibody is Available at Gentaur Genprice with the fastest delivery.

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Product Type: Polyclonal Antibody

Target Names: BUB1B

Aliases: Mitotic checkpoint serine/threonine-protein kinase BUB1 beta (EC 2.7.11.1) (MAD3/BUB1-related protein kinase) (hBUBR1) (Mitotic checkpoint kinase MAD3L) (Protein SSK1), BUB1B, BUBR1 MAD3L SSK1

Background: Essential component of the mitotic checkpoint. Required for normal mitosis progression. The mitotic checkpoint delays anaphase until all chromosomes are properly attached to the mitotic spindle. One of its checkpoint functions is to inhibit the activity of the anaphase-promoting complex/cyclosome (APC/C) by blocking the binding of CDC20 to APC/C, independently of its kinase activity. The other is to monitor kinetochore activities that depend on the kinetochore motor CENPE. Required for kinetochore localization of CENPE. Negatively regulates PLK1 activity in interphase cells and suppresses centrosome amplification. Also implicated in triggering apoptosis in polyploid cells that exit aberrantly from mitotic arrest. May play a role for tumor suppression.

Isotype: IgG

Conjugate: Non-conjugated

Clonality: Polyclonal

Uniport ID: O60566

Host Species: Rabbit

Species Reactivity: Human

Immunogen: Recombinant Human Mitotic checkpoint serine/threonine-protein kinase BUB1 beta protein (230-394AA)

Immunogen Species: Human

Applications: ELISA, IHC, IF, IP

Tested Applications: ELISA, IHC, IF, IP; Recommended dilution: IHC:1:20-1:200, IF:1:50-1:500, IP:1:200-1:2000

Purification Method: >95%, Protein G purified

Dilution Ratio1: ELISA:1:2000-1:10000

Dilution Ratio2: IHC:1:20-1:200

Dilution Ratio3: IF:1:50-1:500

Dilution Ratio4: IP:1:200-1:2000

Dilution Ratio5:

Dilution Ratio6:

Buffer: Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form: Liquid

Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Initial Research Areas: Cell Biology

Research Areas: Epigenetics & Nuclear Signaling;Cancer;Cell biology

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