ACTA1 Antibody | CSB-RA001205A0HU

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SKU:
CSB-RA001205A0HU
Availability:
3 to 7 Working Days
  • ACTA1 Antibody
  • Western Blot<br />
 Positive WB detected in:Hela whole cell lysate,A549 whole cell lysate,Raw264.7 whole cell lysate,NIH/3T3 whole cell lysate,HepG2 whole cell lysate,Rat brain tissue,Rat heart tissue<br />
 All lanes:Actin antibody at 0.95µg/ml<br />
 Secondary<br />
 Goat polyclonal to rabbit IgG at 1/50000 dilution<br />
 Predicted band size: 42 KDa<br />
 Observed band size: 42 KDa<br />
  • IHC image of CSB-RA001205A0HU diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
  • Immunofluorescence staining of HepG2 cells with CSB-RA001205A0HU at 1:60,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
  • Overlay histogram showing Hela cells stained with CSB-RA001205A0HU (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min.The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4℃.The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4℃. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.
€373.00 - €555.00

Description

ACTA1 Antibody | CSB-RA001205A0HU | Cusabio

ACTA1 Antibody is Available at Gentaur Genprice with the fastest delivery.

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Antibody Type: Recombinant Antibody

Target Names: ACTA1

Aliases: Actin, alpha skeletal muscle, Alpha-actin-1, ACTA1, ACTA

Background: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

Isotype: Rabbit IgG

Conjugate: Non-conjugated

Clonality: Monoclonal

Clone Number: 2,50E+04

Uniport ID: P68133

Modified: Tags & Cell Markers

species: Human

Species Reactivity: Human

Immunogen: A synthesized peptide

Tested Applications: ELISA, WB, IHC, IF, FC; Recommended dilution: WB:1:500-1:5000, IHC:1:50-1:500, IF:1:30-1:200

Purification Method: Affinity-chromatography

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Form: Liquid

Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

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