Acetyl-HIST1H3A (K4) Antibody | CSB-PA010418OA04acHU

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CSB-PA010418OA04acHU
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3 to 7 Working Days
  • Acetyl-HIST1H3A (K4) Antibody
  • Immunofluorescence staining of Hela cells with CSB-PA010418OA04acHU at 1:7.5, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L) .
  • Chromatin Immunoprecipitation Hela (4*10<sup>6</sup>, treated with 30mM sodium butyrate for 4h) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5µg anti-HIST1H3A (CSB-PA010418OA04acHU) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter.
  • Western Blot<br />
 Positive WB detected in: Jurkat whole cell lysate, 293 whole cell lysate, HepG2 whole cell lysate (treated by 30mM sodium butyrate for 4h) <br />
 All lanes: HIST1H3A antibody at 0.935µg/ml<br />
 Secondary<br />
 Goat polyclonal to rabbit IgG at 1/50000 dilution<br />
 Predicted band size: 16 kDa<br />
 Observed band size: 16 kDa<br />
  • Immunoprecipitating HIST1H3A in HepG2 whole cell lysate (treated with 30mM sodium butyrate for 4h) <br />
 Lane 1: Rabbit control IgG instead of CSB-PA010418OA04acHU in HepG2 whole cell lysate (treated with 30mM sodium butyrate for 4h) .
 For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) <br />
 Lane 2: CSB-PA010418OA04acHU (5µg) + HepG2 whole cell lysate (treated with 30mM sodium butyrate for 4h) (500µg) <br />
 Lane 3: HepG2 whole cell lysate (treated with 30mM sodium butyrate for 4h) (20µg) <br />
€327.50 - €514.70

Description

Acetyl-HIST1H3A (K4) Antibody | CSB-PA010418OA04acHU | Cusabio

Acetyl-HIST1H3A (K4) Antibody is Available at Gentaur Genprice with the fastest delivery.

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Product Type: Polyclonal Antibody

Target Names: HIST1H3A

Aliases: Histone H3.1 (Histone H3/a) (Histone H3/b) (Histone H3/c) (Histone H3/d) (Histone H3/f) (Histone H3/h) (Histone H3/i) (Histone H3/j) (Histone H3/k) (Histone H3/l), HIST1H3A; HIST1H3B; HIST1H3C; HIST1H3D; HIST1H3E; HIST1H3F; HIST1H3G; HIST1H3H; HIST1H3I; HIST1H3J, H3FA; H3FL; H3FC; H3FB; H3FD; H3FI; H3FH; H3FK; H3FF; H3FJ

Background: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Isotype: IgG

Conjugate: Non-conjugated

Clonality: Polyclonal

Uniport ID: P68431

Host Species: Rabbit

Species Reactivity: Human

Immunogen: Peptide sequence around site of Acetyl-Lys (4) derived from Human Histone H3.1

Immunogen Species: Human

Applications: ELISA, WB, IF, IP, ChIP

Tested Applications: ELISA, WB, IF, IP, ChIP; Recommended dilution: WB:1:200-1:2000, IF:1:1-1:10, IP:1:200-1:2000

Purification Method: Antigen Affinity Purified

Dilution Ratio1: ELISA:1:2000-1:10000

Dilution Ratio2: WB:1:200-1:2000

Dilution Ratio3: IF:1:1-1:10

Dilution Ratio4: IP:1:200-1:2000

Dilution Ratio5:

Dilution Ratio6:

Buffer: Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form: Liquid

Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Initial Research Areas: Epigenetics and Nuclear Signaling

Research Areas: Epigenetics & Nuclear Signaling

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