WT1 Antibody | CSB-RA950156A0HU

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CSB-RA950156A0HU
Availability:
3 to 7 Working Days
  • WT1 Antibody
  • Western Blot<br />
 Positive WB detected in: K562 whole cell lysate, 293T whole cell lysate, A549 whole cell lysate, HEK293 whole cell lysate<br />
 All lanes: Wilms Tumor Protein antibody at 1:1000<br />
 Secondary<br />
 Goat polyclonal to rabbit IgG at 1/50000 dilution<br />
 Predicted band size: 50, 48, 48, 49, 35, 57, 56, 34 kDa<br />
 Observed band size: 50 kDa<br />
  • IHC image of CSB-RA950156A0HU diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
€373.00 - €555.00
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Description

WT1 Antibody | CSB-RA950156A0HU | Cusabio

WT1 Antibody is Available at Gentaur Genprice with the fastest delivery.

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Antibody Type: Recombinant

Target Names: WT1

Aliases: Wilms tumor protein (WT33), WT1

Background: Transcription factor that plays an important role in cellular development and cell survival (PubMed:7862533). Recognizes and binds to the DNA sequence 5'-GCG(T/G)GGGCG-3' (PubMed:7862533, PubMed:17716689, PubMed:25258363). Regulates the expression of numerous target genes, including EPO. Plays an essential role for development of the urogenital system. It has a tumor suppressor as well as an oncogenic role in tumor formation. Function may be isoform-specific: isoforms lacking the KTS motif may act as transcription factors (PubMed:15520190). Isoforms containing the KTS motif may bind mRNA and play a role in mRNA metabolism or splicing (PubMed:16934801). Isoform 1 has lower affinity for DNA, and can bind RNA (PubMed:19123921).

Isotype: Rabbit IgG

Conjugate: Non-conjugated

Clonality: Monoclonal

Clone Number: 5F2

Uniport ID: P19544

Modified: Epigenetics and Nuclear Signaling; Cancer; Developmental biology; Tags & Cell Markers

species: Homo sapiens (Human)

Species Reactivity: Human

Immunogen: A synthesized peptide derived from human Wilms Tumor Protein

Tested Applications: ELISA, WB, IHC; Recommended dilution: WB:1:500-1:5000, IHC:1:50-1:200

Purification Method: Affinity-chromatography

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Form: Liquid

Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

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