TPT1 Antibody | CSB-RA214107A0HU

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3 to 7 Working Days
  • TPT1 Antibody
  • Western Blot<br />
 Positive WB detected in: Hela whole cell lysate, Jurkat whole cell lysate, MCF-7 whole cell lysate, RAW264.7 whole cell lysate, HepG2 whole cell lysate, PC-3 whole cell lysate, Raji whole cell lysate, 293 whole cell lysate, Mouse liver tissue<br />
 All lanes: TPT1 antibody at 1:2000<br />
 Secondary<br />
 Goat polyclonal to rabbit IgG at 1/50000 dilution<br />
 Predicted band size: 20, 16 kDa<br />
 Observed band size: 25 kDa<br />
  • IHC image of CSB-RA214107A0HU diluted at 1:100 and staining in paraffin-embedded human prostate tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
  • Immunofluorescence staining of SY5Y Cells with CSB-RA214107A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).
  • Immunoprecipitating TCTP in Hela whole cell lysate<br />
 Lane 1: Rabbit control IgG instead of CSB-RA214107A0HU in Hela whole cell lysate.
 For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)<br />
 Lane 2: CSB-RA214107A0HU(2µg)+ Hela whole cell lysate(500µg)<br />
 Lane 3: Hela whole cell lysate (10µg)<br />
€373.00 - €555.00
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TPT1 Antibody | CSB-RA214107A0HU | Cusabio

TPT1 Antibody is Available at Gentaur Genprice with the fastest delivery.

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Antibody Type: Recombinant

Target Names: TPT1

Aliases: Translationally-controlled tumor protein (TCTP) (Fortilin) (Histamine-releasing factor) (HRF) (p23), TPT1

Background: Involved in calcium binding and microtubule stabilization.

Isotype: Rabbit IgG

Conjugate: Non-conjugated

Clonality: Monoclonal

Clone Number: 10A9

Uniport ID: P13693

Modified: Immunology

species: Homo sapiens (Human)

Species Reactivity: Human, Mouse

Immunogen: A synthesized peptide derived from human TCTP

Tested Applications: ELISA, WB, IHC, IF, IP; Recommended dilution: WB:1:500-1:5000, IHC:1:50-1:200, IF:1:20-1:200, IP:1:200-1:1000

Purification Method: Affinity-chromatography

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Form: Liquid

Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

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