Recombinant Xanthomonas oryzae pv. oryzae Elongation factor Tu (tuf) | CSB-EP2860XBC

Recombinant Xanthomonas oryzae pv. oryzae Elongation factor Tu (tuf) | CSB-EP2860XBC | Cusabio

Alternative Name(s): EF-Tu

Gene Names: tuf

Research Areas: Epigenetics and Nuclear Signaling

Organism: Xanthomonas oryzae pv. oryzae (strain PXO99A)

AA Sequence: MNVGTIGHVDHGKTTLTAALTKIGAERFGGEFKAYDAIDAAPEEKARGITISTAHVEYESPSRHYAHVDCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHILLSRQVGVPHIVVFLNKADMVDDAELLELVEMEVRELLSKYDFPGDDTPIIHGSARLALDGDQSEIGVPAILKLVDALDTFIPEPTRDVDRPFLMPVEDVFSISGRGTVVTGRIERGIIKVGDEIEIVGIRATQKTTVTGVEMFRKLLDQGQAGDNAGLLLRGTKRDDVERGQVLCKPGSIKPHTEFEAEVYVLSKDEGGRHTPFFKGYRPQLYFRTTDITGAIDLPEGVEMVMPGDNVKMTVTLINPVAMDEGLRFAIREGGRTVGAGVVSKIIK

Source: E.coli

Tag Info: N-terminal 10xHis-tagged

Expression Region: 1-384aa

Sequence Info: Full Length

MW: 47.7 kDa

Purity: Greater than 85% as determined by SDS-PAGE.

Relevance: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.

Reference: "Genome sequence and rapid evolution of the rice pathogen Xanthomonas oryzae pv. oryzae PXO99A." Salzberg S.L., Sommer D.D., Schatz M.C., Phillippy A.M., Rabinowicz P.D., Tsuge S., Furutani A., Ochiai H., Delcher A.L., Kelley D., Madupu R., Puiu D., Radune D., Shumway M., Trapnell C., Aparna G., Jha G., Pandey A. Bogdanove A.J. BMC Genomics 9:204-204(2008)

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.

Function:

Involvement in disease:

Subcellular Location:

Protein Families:

Tissue Specificity:

Paythway:

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Uniprot ID: A0A0J9WWH0

HGNC Database Link: N/A

UniGene Database Link: N/A

KEGG Database Link: N/A

STRING Database Link: N/A

OMIM Database Link: N/A