Recombinant Ustilago sphaerogena Ribonuclease U2 (RNU2) | CSB-EP360432UBD

Recombinant Ustilago sphaerogena Ribonuclease U2 (RNU2) | CSB-EP360432UBD | Cusabio

Alternative Name(s): RNU2; Ribonuclease U2; RNase U2; EC 4.6.1.20

Gene Names: RNU2

Research Areas: Others

Organism: Ustilago sphaerogena (Smut fungus)

AA Sequence: CDIPQSTNCGGNVYSNDDINTAIQGALDDVANGDRPDNYPHQYYDEASEDITLCCGSGPWSEFPLVYNGPYYSSRDNYVSPGPDRVIYQTNTGEFCATVTHTGAASYDGFTQCS

Source: E.coli

Tag Info: N-terminal 6xHis-B2M-tagged

Expression Region: 1-114aa

Sequence Info: Full Length

MW: 26.4 kDa

Purity: Greater than 85% as determined by SDS-PAGE.

Relevance: After treatment by base Asn-32 and Asp-45 partially isomerise by succinimide rearrangement to form iosaspartyl peptides.

Reference: "Ribonuclease U2: cloning, production in Pichia pastoris and affinity chromatography purification of the active recombinant protein." Martinez-Ruiz A., Garcia-Ortega L., Kao R., Onaderra M., Mancheno J.M., Davies J., Martinez del Pozo A., Gavilanes J.G. FEMS Microbiol. Lett. 189:165-169(2000)

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.

Function:

Involvement in disease:

Subcellular Location:

Protein Families: Ribonuclease U2 family

Tissue Specificity:

Paythway:

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Uniprot ID: P00654

HGNC Database Link: N/A

UniGene Database Link: N/A

KEGG Database Link: N/A

STRING Database Link: N/A

OMIM Database Link: N/A