Recombinant Plasmodium falciparum Multidrug resistance protein (MDR1), partial | CSB-EP318386PLT
- 3 - 7 Working Days
Recombinant Plasmodium falciparum Multidrug resistance protein (MDR1), partial | CSB-EP318386PLT | Cusabio
Alternative Name(s): Chloroquine resistance protein
Gene Names: MDR1
Research Areas: Others
Organism: Plasmodium falciparum (isolate FC27 / Papua New Guinea)
AA Sequence: KGDSENAKLSFEKYYPLMIRKSNIDVRDDGGIRINKNLIKGKVDIKDVNFRYISRPNVPIYKNLSFTCDSKKTTAIVGETGSGKSTFMNLLLRFYDLKNDHIILKNDMTNFQDYQNNNNNSLVLKNVNEFSNQSGSAEDYTVFNNNGEILLDDINICDYNLRDLRNLFSIVSQEPMLFNMSIYENIKFGREDATLEDVKRVSKFAAIDEFIESLPNKYDTNVGPYGKSLSGGQKQRIAIARALLREPKILLLDEATSSLDSNSEKLIEKTIVDIKDKADKTIITIAHRIASIKRSDKIVVFNNPDRNGTFVQSHGTHDELLSAQDGIYKKYVKLAK
Tag Info: N-terminal 6xHis-SUMO-tagged
Expression Region: 1084-1419aa
Sequence Info: Cytoplasmic Domain
MW: 54.2 kDa
Purity: Greater than 90% as determined by SDS-PAGE.
Relevance: Energy-dependent efflux pump responsible for decreased drug accumulation in multidrug-resistant cells.
Reference: Amplification of the multidrug resistance gene in some chloroquine-resistant isolates of P. falciparum.Foote S.J., Thompson J.K., Cowman A.F., Kemp D.J.Cell 57:921-930(1989)
Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.
Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.
Function: Energy-dependent efflux pump responsible for decreased drug accumulation in multidrug-resistant cells.
Involvement in disease:
Subcellular Location: Membrane, Multi-pass membrane protein
Protein Families: ABC transporter superfamily, ABCB family, Multidrug resistance exporter (TC 3.A.1.201) subfamily
Form: Liquid or Lyophilized powder
Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Uniprot ID: P13568
HGNC Database Link: N/A
UniGene Database Link: N/A
KEGG Database Link: N/A
STRING Database Link: N/A
OMIM Database Link: N/A