Recombinant Mouse Tryptase (Tpsab1), partial | CSB-YP024127MO
- 25 - 35 Working Days
Recombinant Mouse Tryptase (Tpsab1), partial | CSB-YP024127MO | Cusabio
Alternative Name(s): Mast cell protease 7 ;mMCP-7Tryptase alpha/beta-1
Gene Names: Tpsab1
Research Areas: Others
Organism: Mus musculus (Mouse)
AA Sequence: AHGNKWPWQVSLRANDTYWMHFCGGSLIHPQWVLTAAHCVGPDVADPNKVRVQLRKQYLYYHDHLMTVSQIITHPDFYIVQDGADIALLKLTNPVNISDYVHPVPLPPASETFPSGTLCWVTGWGNIDNGVNLPPPFPLKEVQVPIIENHLCDLKYHKGLITGDNVHIVRDDMLCAGNEGHDSCQGDSGGPLVCKVEDTWLQAGVVSWGEGCAQPNRPGIYTRVTYYLDWIHH
Tag Info: N-terminal 6xHis-tagged
Expression Region: 35-267aa
Sequence Info: Partial
MW: 28.1 kDa
Purity: Greater than 90% as determined by SDS-PAGE.
Relevance: Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type.
Reference: Isolation, characterization, and transcription of the gene encoding mouse mast cell protease 7.McNeil H.P., Reynolds D.S., Schiller V., Ghildyal N., Gurley D.S., Austen K.F., Stevens R.L.Proc. Natl. Acad. Sci. U.S.A. 89:11174-11178(1992)
Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.
Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.
Function: Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type. May play a role in innate immunity (By similarity).
Involvement in disease:
Protein Families: Peptidase S1 family, Tryptase subfamily
Form: Liquid or Lyophilized powder
Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Uniprot ID: Q02844
HGNC Database Link: N/A
UniGene Database Link: UniGene
KEGG Database Link: KEGG
STRING Database Link: STRING
OMIM Database Link: N/A