Recombinant Human Melanoma-derived growth regulatory protein (MIA) (Active) | CSB-AP000431HU

Recombinant Human Melanoma-derived growth regulatory protein (MIA) (Active) | CSB-AP000431HU | Cusabio

Protein Description: Full Length of Mature Protein

Alternative Name (s) : Melanoma inhibitory activity protein,

Gene Names: MIA

Research Areas: Immunology

Species: Homo sapiens (Human)

Source: E.Coli

Tag Info: Tag-Free

Expression Region: 25-131aa

Sequence Info: GPMPKLADRK LCADQECSHP ISMAVALQDY MAPDCRFLTI HRGQVVYVFS KLKGRGRLFW GGSVQGDYYG DLAARLGYFP SSIVREDQTL KPGKVDVKTD KWDFYCQ

Biological Activity: Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human A375 cell line is less than 5 μg/ml, corresponding to a specific activity of ＞ 200 IU/mg.

MW: 12.1 kDa

Purity: >98% as determined by SDS-PAGE and HPLC.

Endotoxin: Less than 1.0 EU/µg as determined by LAL method.

Relevance: Elicits growth inhibition on melanoma cells in vitro as well as some other neuroectodermal tumors, including gliomas.

PubMed ID: 7923218; 8550608; 12230496; 15489334; 15340161; 19807924; 22419821; 11331761

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.

Function: Elicits growth inhibition on melanoma cells in vitro as well as some other neuroectodermal tumors, including gliomas.

Involvement in disease:

Subcellular Location: Secreted

Protein Families: MIA/OTOR family

Tissue Specificity: All malignant melanoma cell lines tested and infrequently in glioma cell lines.

Paythway:

Form: Lyophilized powder

Buffer: Lyophilized from a 0.2 µm filtered 20 mM PB, pH 7.4, 150mM NaCl

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Uniprot ID: Q16674

Uniprot Entry Name: MIA_HUMAN

HGNC Database Link: HGNC

UniGene Database Link: UniGene

KEGG Database Link: KEGG

STRING Database Link: STRING

OMIM Database Link: OMIM