hns Antibody | CSB-PA359764LA01ENV

hns Antibody | CSB-PA359764LA01ENV | Cusabio

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Product Type: Polyclonal Antibody

Target Names: hns

Aliases: DNA-binding protein H-NS, Heat-stable nucleoid-structuring protein, Histone-like protein HLP-II, Protein B1, Protein H1, hns, bglY, cur, drdX, hnsA, msyA, osmZ, pilG, topS

Background: A DNA-binding protein implicated in transcriptional repression (silencing) (PubMed:333393, PubMed:2128918, PubMed:8890170, PubMed:8913298, PubMed:9398522, PubMed:16963779, PubMed:17046956, PubMed:23543115) . Also involved in bacterial chromosome organization and compaction (PubMed:6379600, PubMed:10982869, PubMed:21903814) . H-NS binds tightly to AT-rich dsDNA and inhibits transcription (PubMed:2512122, PubMed:16963779, PubMed:17435766, PubMed:17881364, PubMed:23543115) . Binds upstream and downstream of initiating RNA polymerase, trapping it in a loop and preventing transcription (PubMed:11714691) . Binds to hundreds of sites, approximately half its binding sites are in non-coding DNA, which only accounts for about 10% of the genome (PubMed:16963779, PubMed:17046956, PubMed:23543115) . Many of these loci were horizontally transferred (HTG) ; this offers the selective advantage of silencing foreign DNA while keeping it in the genome in case of need (PubMed:17046956, PubMed:17881364, PubMed:26789284) . Suppresses transcription at many intragenic sites as well as transcription of spurious, non-coding RNAs genome-wide (PubMed:24449106) . Repression of HTG by H-NS is thought to allow their DNA to evolve faster than non-H-NS-bound regions, and facilitates integration of HTG into transcriptional regulatory networks (PubMed:26789284) . A subset of H-NSStpA-regulated genes also require Hha (andor Cnu, ydgT) for repression; Hha and Cnu increase the number of genes DNA bound by H-NSStpA and may also modulate the oligomerization of the H-NSStpA-complex (PubMed:23543115) . The protein forms 2 clusters in the nucleoid which gather hns-bound loci together, bridging non-contiguous DNA, and causes DNA substantial condensation (PubMed:21903814) . Binds DNA better at low temperatures than at 37 degrees Celsius; AT-rich sites nucleate H-NS binding, further DNA-binding is cooperative and this cooperativity decreases with rising temperature (PubMed:17435766, PubMed:17881364) . Transcriptional repression can be inhibited by dominant-negative mutants of StpA or itself (PubMed:8755860) . May effect transcriptional elongation (PubMed:25638302) . Can increase translational efficiency of mRNA with suboptimal Shine-Dalgarno sequences (PubMed:20595230) . Plays a role in the thermal control of pili and adhesive curli fimbriae production, by inducing transcription of csgD (PubMed:17010156) . Plays a role in flagellar function (PubMed:11031114) . Represses the CRISPR-cas promoters, permits only weak transcription of the crRNA precursor; its repression is antagonized by LeuO (PubMed:20132443, PubMed:20659289) . Binds preferentially to the upstream region of its own gene recognizing two segments of DNA on both sides of a bend centered around -150 (PubMed:7934818) . Overexpression suppresses secY24, a temperature-sensitive mutation (PubMed:1537791) . Has also been reported to activate transcription of some genes (PubMed:4566454, PubMed:338303, PubMed:2128918) .

Isotype: IgG

Conjugate: Non-conjugated

Clonality: Polyclonal

Uniport ID: P0ACF8

Host Species: Rabbit

Species Reactivity: Escherichia coli

Immunogen: Recombinant Escherichia coli DNA-binding protein H-NS protein (1-137AA)

Immunogen Species: Escherichia coli (strain K12)

Applications: ELISA, WB

Tested Applications: ELISA, WB; Recommended dilution: WB:1:1000-1:5000

Purification Method: >95%, Protein G purified

Dilution Ratio1: ELISA:1:2000-1:10000

Dilution Ratio2: WB:1:1000-1:5000

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Buffer: Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form: Liquid

Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Initial Research Areas: Others

Research Areas: Others